Authors : Amol C. Vikhe and Janardhan N. Nehul
Page Nos : 657-664
Description :
Creation of genetic variability in crops for improvement in its Qualitative and Quantitative parameters;
mutagens (Chemical and Physical) act as crucial role in the field of plant breeding. Using Ethyl methane
Sulphonate (EMS), Sodium azide (SA) and Gamma radiation (GR) dominant and recessive qualitative as
well as quantitative parameters were studied in this experiment. The research study was carried out on
Vigna radiata Cultivar- Naval. The seeds of cultivar were exposed to treatments with EMS, SA and GR
concentrations of mutagens. The selected concentrations were determined by LD50 Value i.e. 10, 15, 20
mM from EMS; 2, 3, 5 mM from SA and 250, 350, 450 Gy from Gamma radiation. These doses showed
significant results on Quantitative parameters in M2 generation. The yield contributing traits showed
positive and negative correlation against the control. Among these treatments we found some dominant
and recessive mutants in relation with yield contributing and morphological traits which are different
than the control. These mutants were harvested and collected separately for sowing purpose in M3
generation to study their performance in next generation.
